Full Answer (what I think the bullets mean)
Captain of serenity.
Captain Malcolm Reynolds from Firefly, who is also the star in the movie Serenity.
Lover of Polish beast slayer.
Yennefer of Vengerberg, wife of Geralt of Rivia (credit to John Eisbrener) Now I know why I didn't get this one. Never played the Witcher series.
Sister of double victor.
Primrose Everdeen from Hunger games. Credit to Richard.
Foul-mouthed one-eyed soldier.
Richard says it could be Uno Nomesta instead though. He's probably right... Even though I want it to be Mat.
From Richard, "Zakath hated the Murgos"
Owner of runaway bum.
As Richard mentions, "Zack Freeman's bum went running off on its own."
Fateful answerer of "What do you want?"
Londo Mollari from Babylon 5, thanks to Richard again.
Headmaster of Scandinavian school
Igor Karkaroff, as Richard mentioned, is headmaster of Durmstrang. But is Durmstrang in Scandinavia?
Empress of southern rebels.
Nasuada, from Eragon (Credit to Pugmonkey) Now that you mention it... This one makes a lot of sense.
Destroyer of the precious.
Gollum from Lord of the Rings
Victim of black puddle.
As Richard mentions, "Tasha Yar was killed by a black puddle."
Queen of island foxes.
Silth, High Queen of Castle Marl from the Redwall novel Marlfox (Credit to El-Guest)
Lonely pilot with gun.
Blue djinn deputy.
As El-Guest figured out, the blue djinn is Ilwan, from the Never Ending Story.
The last centurion.
Rory Williams from Doctor Who
Husband of fallen star.
As Richard says, Tristan married Yvaine at the end of Stardust.
As Richard mentions (he really should get the credit) this spells out
MY PUZZLING T SHIRT = My Puzzling T-Shirt